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neuronal nuclei  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc neuronal nuclei
    Neuronal Nuclei, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuronal+nuclei/NeuN+XP+Rabbit+mAb/pmc12296469-67-19-24
    Average 97 stars, based on 560 article reviews
    neuronal nuclei - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: Identification of the target proteins was accomplished using a DAB Detection Immunohistochemistry Kit (Abcam, Waltham, MA, USA, Cat# ab64264). .. Each section was first blocked using a blocking solution and subsequently incubated overnight at 4°C with primary antibodies targeting neuronal nuclei (NeuN, rabbit, 1:200, Cell Signaling Technology, Cat# 24307, RRID: AB_2651140), glial fibrillary acidic protein (GFAP, rabbit, 1:200; Cell Signaling Technology, Cat# 12389, RRID: AB_2631098), and ionized calcium binding adaptor molecule-1 (Iba1, rabbit, 1:800; Cell Signaling Technology, Cat# 17198, RRID: AB_2820254). ..

    Article Title: Methanolic extract of Korean Red ginseng attenuates NLRP3 inflammasome-mediated neuroinflammation in chronic cerebral hypoperfusion-induced cognitive impairment: A comparative study.
    Article Snippet: Korean ginseng is classified into White or Red ginseng based on its processing methods.. Traditional Korean medicine recognizes the differing effects and medicinal properties between White or Red ginseng.. This study aimed to compare the effects and mechanisms of action of methanolic extracts of White ginseng and Red ginseng (WG and RG, respectively) in a vascular dementia (VaD) mouse model.

    Article Title: Effect of Water Temperature during Aquatic Exercise on Cognitive Impairment in Mice with Chronic Cerebral Hypoperfusion
    Article Snippet: .. After drying and reacting with blocking buffer (5% BSA) for 1 h at 25°C, the brain tissue slices were rinsed, and other materials such as neuronal nuclei (Cat No. 94403, Cell Signaling Technology, Danvers, MA, USA), cluster of differentiation 68 (CD68; Cat No. ab283654, Abcam, Cambridge, UK), glial fibrillary acidic protein (GFAP; Cat No. 12389, Cell Signaling Technology), tumor necrosis factor-α (TNF-α; Cat No. ab183218, Abcam), and myelin-associated glycoprotein (MAG; Cat No. ab277524; Abcam) and myelin basic protein (MBP; Cat No. ab313827; Abcam, Cambridge, UK) antibodies were added. ..

    Article Title: Effect of Water Temperature during Aquatic Exercise on Cognitive Impairment in Mice with Chronic Cerebral Hypoperfusion.
    Article Snippet: A cryostat (Leica, Wetzlar, Germany) was then used to create brain sections that were 25 μm thick. .. Sections were kept in a refrigerator at −80°C until they were needed after being on glass slides for 12 h. Brain tissue immunofluorescence staining After drying and reacting with blocking buffer (5% BSA) for 1 h at 25°C, the brain tissue slices were rinsed, and other materials such as neuronal nuclei (NeuN, Cat no. 94403, Cell Signaling Technology, Danvers, MA, USA), cluster of differentiation 68 (CD68, Cat no. ab283654, Abcam, Cambridge, UK), glial fibrillary acidic protein (GFAP, Cat no. 12389, Cell Signaling Technology), tumor necrosis factor-α (TNF-α, Cat no. ab183218, Abcam) and myelin-associated glycoprotein (MAG; Cat No. ab277524; Abcam) and myelin basic protein (MBP; Cat No. ab313827; Abcam, Cambridge, UK) antibodies were added. ..

    Article Title: Assessing the effects of aging on the Koizumi’s and Zea Longa’s methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study
    Article Snippet: The brain slices (with a thickness of 25 μm) from each mouse were placed on a glass slide using a cryostat (CM1850, Leica, Wetzlar, Germany) and kept in a refrigerator at −80°C until needed. .. After drying and reacting with blocking buffer (5% BSA) for 1 hour at 25°C, the brain tissue slices were rinsed, and other materials such as NOD-like receptor protein 3 (NLRP3, Cat no. ab270449, Abcam, Cambridge, UK), cyclin-dependent kinase 5 (CDK5, Abcam, ab40773), cytosolic phospholipase A 2 (cPLA 2 , Abcam, ab227246), neuronal nuclei (NeuN, Cat no. 94403, Cell Signaling Technology, Danvers, MA, USA), glial fibrillary acidic protein (GFAP, Cat no. 12389, Cell Signaling Technology) antibodies, and others were added. ..

    Incubation:

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: Identification of the target proteins was accomplished using a DAB Detection Immunohistochemistry Kit (Abcam, Waltham, MA, USA, Cat# ab64264). .. Each section was first blocked using a blocking solution and subsequently incubated overnight at 4°C with primary antibodies targeting neuronal nuclei (NeuN, rabbit, 1:200, Cell Signaling Technology, Cat# 24307, RRID: AB_2651140), glial fibrillary acidic protein (GFAP, rabbit, 1:200; Cell Signaling Technology, Cat# 12389, RRID: AB_2631098), and ionized calcium binding adaptor molecule-1 (Iba1, rabbit, 1:800; Cell Signaling Technology, Cat# 17198, RRID: AB_2820254). ..

    Binding Assay:

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: Identification of the target proteins was accomplished using a DAB Detection Immunohistochemistry Kit (Abcam, Waltham, MA, USA, Cat# ab64264). .. Each section was first blocked using a blocking solution and subsequently incubated overnight at 4°C with primary antibodies targeting neuronal nuclei (NeuN, rabbit, 1:200, Cell Signaling Technology, Cat# 24307, RRID: AB_2651140), glial fibrillary acidic protein (GFAP, rabbit, 1:200; Cell Signaling Technology, Cat# 12389, RRID: AB_2631098), and ionized calcium binding adaptor molecule-1 (Iba1, rabbit, 1:800; Cell Signaling Technology, Cat# 17198, RRID: AB_2820254). ..

    Staining:

    Article Title: Integrated Profiling of DEHP-Induced Hippocampal Neurotoxicity in Adult Female Rats Based on Transcriptomic and Neurobiological Analyses.
    Article Snippet: .. The antibodies used in IF Staining were as follows: Cytochrome c oxidase subunit 4 isoform 1 (COX4I1; 1:50; 66110; Proteintech Group, Inc., Rosemont, IL, USA), Superoxide dismutase 2 (SOD2; 1:50; sc-18503; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), 8-hydroxy2′-deoxyguanosine (8-OHdG; 1:300; sc-65385; Santa Cruz Biotechnology), 3-nitrotyrosine (3-NT; 1:50; sc-32757; Santa Cruz Biotechnology), NAD(P)H quinone dehydrogenase 1 (NQO1; 1:50; sc-376023; Santa Cruz Biotechnology), Ionized calcium-binding adapter molecule 1 (Iba1; 1:50; NB100-1028; Novus Biologicals, Centennial, CO, USA), Complement component 3 (C3; 1:100; 21337-1-AP; Proteintech Group), Glial fibrillary acidic protein (GFAP; 1:1000; ab53554; Abcam, Cambridge, UK), SRY-box transcription factor 2 (SOX2; 1:500; ab97959; Abcam), Doublecortin (DCX; 1:50; sc-8066; Santa Cruz Biotechnology), Neuronal nuclei (NeuN; 1:100; 24307; Cell Signaling Technology, Danvers, MA, USA), Oligodendrocyte transcription factor 1 (Olig1; 1:50; GTX104823; GeneTex, Inc., Irvine, CA, USA), Oligodendrocyte transcription factor 2 (Olig2; 1:50; GTX132733; GeneTex), Proteolipid protein 1 (PLP1; 1:100; HA500202; Huabio, Hangzhou, China), p-tau231 (1:50; AP005; ABclonal Technology, Woburn, MA, USA), p-tau396 (1:100; AP1028; ABclonal Technology), Vesicle-associated membrane protein 2 (VAMP2; 1:250; ab181869; Abcam), Syntaxin (1:500; ab3265; Abcam), Synapse-associated protein 102 (SAP102; 1:30; PA1-045; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Microtubule-associated protein 2 (MAP2; 1:50; sc-56561; Santa Cruz Biotechnology), Calcium/calmodulin-dependent protein kinase II α (CaMKIIα; 1:50; 50049; Cell Signaling Technology), Spinophilin (SPN; 1:500; 14136; Cell Signaling Technology), Alexa fluorTM488 donkey anti-rabbit IgG (1:250; A21206; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Alexa fluorTM 488 donkey anti-mouse IgG (1:250; A21202; Invitrogen), Alexa fluorTM 594 donkey antirabbit IgG (1:250; A21207; Invitrogen), Alexa fluorTM 594 donkey anti-mouse IgG (1:250; A21203; Invitrogen). .. Hippocampal tissues were homogenized in ice-cold RIPA lysis buffer (HY-K1001; MedChemExpress, Monmouth Junction, NJ, USA) with a 10% protease inhibitor cocktail (HY-K0010; MedChemExpress).

    Article Title: Effect of Water Temperature during Aquatic Exercise on Cognitive Impairment in Mice with Chronic Cerebral Hypoperfusion.
    Article Snippet: A cryostat (Leica, Wetzlar, Germany) was then used to create brain sections that were 25 μm thick. .. Sections were kept in a refrigerator at −80°C until they were needed after being on glass slides for 12 h. Brain tissue immunofluorescence staining After drying and reacting with blocking buffer (5% BSA) for 1 h at 25°C, the brain tissue slices were rinsed, and other materials such as neuronal nuclei (NeuN, Cat no. 94403, Cell Signaling Technology, Danvers, MA, USA), cluster of differentiation 68 (CD68, Cat no. ab283654, Abcam, Cambridge, UK), glial fibrillary acidic protein (GFAP, Cat no. 12389, Cell Signaling Technology), tumor necrosis factor-α (TNF-α, Cat no. ab183218, Abcam) and myelin-associated glycoprotein (MAG; Cat No. ab277524; Abcam) and myelin basic protein (MBP; Cat No. ab313827; Abcam, Cambridge, UK) antibodies were added. ..

    Article Title: Integrated Profiling of DEHP-Induced Hippocampal Neurotoxicity in Adult Female Rats Based on Transcriptomic and Neurobiological Analyses
    Article Snippet: .. The antibodies used in IF Staining were as follows: Cytochrome c oxidase subunit 4 isoform 1 (COX4I1; 1:50; 66110; Proteintech Group, Inc., Rosemont, IL, USA), Superoxide dismutase 2 (SOD2; 1:50; sc-18503; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), 8-hydroxy-2′-deoxyguanosine (8-OHdG; 1:300; sc-65385; Santa Cruz Biotechnology), 3-nitrotyrosine (3-NT; 1:50; sc-32757; Santa Cruz Biotechnology), NAD(P)H quinone dehydrogenase 1 (NQO1; 1:50; sc-376023; Santa Cruz Biotechnology), Ionized calcium-binding adapter molecule 1 (Iba1; 1:50; NB100-1028; Novus Biologicals, Centennial, CO, USA), Complement component 3 (C3; 1:100; 21337-1-AP; Proteintech Group), Glial fibrillary acidic protein (GFAP; 1:1000; ab53554; Abcam, Cambridge, UK), SRY-box transcription factor 2 (SOX2; 1:500; ab97959; Abcam), Doublecortin (DCX; 1:50; sc-8066; Santa Cruz Biotechnology), Neuronal nuclei (NeuN; 1:100; 24307; Cell Signaling Technology, Danvers, MA, USA), Oligodendrocyte transcription factor 1 (Olig1; 1:50; GTX104823; GeneTex, Inc., Irvine, CA, USA), Oligodendrocyte transcription factor 2 (Olig2; 1:50; GTX132733; GeneTex), Proteolipid protein 1 (PLP1; 1:100; HA500202 ; Huabio, Hangzhou, China), p-tau231 (1:50; AP005; ABclonal Technology, Woburn, MA, USA), p-tau396 (1:100; AP1028; ABclonal Technology), Vesicle-associated membrane protein 2 (VAMP2; 1:250; ab181869; Abcam), Syntaxin (1:500; ab3265; Abcam), Synapse-associated protein 102 (SAP102; 1:30; PA1-045; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Microtubule-associated protein 2 (MAP2; 1:50; sc-56561; Santa Cruz Biotechnology), Calcium/calmodulin-dependent protein kinase II α (CaMKIIα; 1:50; 50049; Cell Signaling Technology), Spinophilin (SPN; 1:500; 14136; Cell Signaling Technology), Alexa fluorTM488 donkey anti-rabbit IgG (1:250; A21206; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Alexa fluorTM 488 donkey anti-mouse IgG (1:250; A21202; Invitrogen), Alexa fluorTM 594 donkey anti-rabbit IgG (1:250; A21207; Invitrogen), Alexa fluorTM 594 donkey anti-mouse IgG (1:250; A21203; Invitrogen). .. Hippocampal tissues were homogenized in ice-cold RIPA lysis buffer (HY-K1001; MedChemExpress, Monmouth Junction, NJ, USA) with a 10% protease inhibitor cocktail (HY-K0010; MedChemExpress).

    Membrane:

    Article Title: Integrated Profiling of DEHP-Induced Hippocampal Neurotoxicity in Adult Female Rats Based on Transcriptomic and Neurobiological Analyses.
    Article Snippet: .. The antibodies used in IF Staining were as follows: Cytochrome c oxidase subunit 4 isoform 1 (COX4I1; 1:50; 66110; Proteintech Group, Inc., Rosemont, IL, USA), Superoxide dismutase 2 (SOD2; 1:50; sc-18503; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), 8-hydroxy2′-deoxyguanosine (8-OHdG; 1:300; sc-65385; Santa Cruz Biotechnology), 3-nitrotyrosine (3-NT; 1:50; sc-32757; Santa Cruz Biotechnology), NAD(P)H quinone dehydrogenase 1 (NQO1; 1:50; sc-376023; Santa Cruz Biotechnology), Ionized calcium-binding adapter molecule 1 (Iba1; 1:50; NB100-1028; Novus Biologicals, Centennial, CO, USA), Complement component 3 (C3; 1:100; 21337-1-AP; Proteintech Group), Glial fibrillary acidic protein (GFAP; 1:1000; ab53554; Abcam, Cambridge, UK), SRY-box transcription factor 2 (SOX2; 1:500; ab97959; Abcam), Doublecortin (DCX; 1:50; sc-8066; Santa Cruz Biotechnology), Neuronal nuclei (NeuN; 1:100; 24307; Cell Signaling Technology, Danvers, MA, USA), Oligodendrocyte transcription factor 1 (Olig1; 1:50; GTX104823; GeneTex, Inc., Irvine, CA, USA), Oligodendrocyte transcription factor 2 (Olig2; 1:50; GTX132733; GeneTex), Proteolipid protein 1 (PLP1; 1:100; HA500202; Huabio, Hangzhou, China), p-tau231 (1:50; AP005; ABclonal Technology, Woburn, MA, USA), p-tau396 (1:100; AP1028; ABclonal Technology), Vesicle-associated membrane protein 2 (VAMP2; 1:250; ab181869; Abcam), Syntaxin (1:500; ab3265; Abcam), Synapse-associated protein 102 (SAP102; 1:30; PA1-045; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Microtubule-associated protein 2 (MAP2; 1:50; sc-56561; Santa Cruz Biotechnology), Calcium/calmodulin-dependent protein kinase II α (CaMKIIα; 1:50; 50049; Cell Signaling Technology), Spinophilin (SPN; 1:500; 14136; Cell Signaling Technology), Alexa fluorTM488 donkey anti-rabbit IgG (1:250; A21206; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Alexa fluorTM 488 donkey anti-mouse IgG (1:250; A21202; Invitrogen), Alexa fluorTM 594 donkey antirabbit IgG (1:250; A21207; Invitrogen), Alexa fluorTM 594 donkey anti-mouse IgG (1:250; A21203; Invitrogen). .. Hippocampal tissues were homogenized in ice-cold RIPA lysis buffer (HY-K1001; MedChemExpress, Monmouth Junction, NJ, USA) with a 10% protease inhibitor cocktail (HY-K0010; MedChemExpress).

    Article Title: Integrated Profiling of DEHP-Induced Hippocampal Neurotoxicity in Adult Female Rats Based on Transcriptomic and Neurobiological Analyses
    Article Snippet: .. The antibodies used in IF Staining were as follows: Cytochrome c oxidase subunit 4 isoform 1 (COX4I1; 1:50; 66110; Proteintech Group, Inc., Rosemont, IL, USA), Superoxide dismutase 2 (SOD2; 1:50; sc-18503; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), 8-hydroxy-2′-deoxyguanosine (8-OHdG; 1:300; sc-65385; Santa Cruz Biotechnology), 3-nitrotyrosine (3-NT; 1:50; sc-32757; Santa Cruz Biotechnology), NAD(P)H quinone dehydrogenase 1 (NQO1; 1:50; sc-376023; Santa Cruz Biotechnology), Ionized calcium-binding adapter molecule 1 (Iba1; 1:50; NB100-1028; Novus Biologicals, Centennial, CO, USA), Complement component 3 (C3; 1:100; 21337-1-AP; Proteintech Group), Glial fibrillary acidic protein (GFAP; 1:1000; ab53554; Abcam, Cambridge, UK), SRY-box transcription factor 2 (SOX2; 1:500; ab97959; Abcam), Doublecortin (DCX; 1:50; sc-8066; Santa Cruz Biotechnology), Neuronal nuclei (NeuN; 1:100; 24307; Cell Signaling Technology, Danvers, MA, USA), Oligodendrocyte transcription factor 1 (Olig1; 1:50; GTX104823; GeneTex, Inc., Irvine, CA, USA), Oligodendrocyte transcription factor 2 (Olig2; 1:50; GTX132733; GeneTex), Proteolipid protein 1 (PLP1; 1:100; HA500202 ; Huabio, Hangzhou, China), p-tau231 (1:50; AP005; ABclonal Technology, Woburn, MA, USA), p-tau396 (1:100; AP1028; ABclonal Technology), Vesicle-associated membrane protein 2 (VAMP2; 1:250; ab181869; Abcam), Syntaxin (1:500; ab3265; Abcam), Synapse-associated protein 102 (SAP102; 1:30; PA1-045; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Microtubule-associated protein 2 (MAP2; 1:50; sc-56561; Santa Cruz Biotechnology), Calcium/calmodulin-dependent protein kinase II α (CaMKIIα; 1:50; 50049; Cell Signaling Technology), Spinophilin (SPN; 1:500; 14136; Cell Signaling Technology), Alexa fluorTM488 donkey anti-rabbit IgG (1:250; A21206; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), Alexa fluorTM 488 donkey anti-mouse IgG (1:250; A21202; Invitrogen), Alexa fluorTM 594 donkey anti-rabbit IgG (1:250; A21207; Invitrogen), Alexa fluorTM 594 donkey anti-mouse IgG (1:250; A21203; Invitrogen). .. Hippocampal tissues were homogenized in ice-cold RIPA lysis buffer (HY-K1001; MedChemExpress, Monmouth Junction, NJ, USA) with a 10% protease inhibitor cocktail (HY-K0010; MedChemExpress).

    Immunofluorescence:

    Article Title: Effect of Water Temperature during Aquatic Exercise on Cognitive Impairment in Mice with Chronic Cerebral Hypoperfusion.
    Article Snippet: A cryostat (Leica, Wetzlar, Germany) was then used to create brain sections that were 25 μm thick. .. Sections were kept in a refrigerator at −80°C until they were needed after being on glass slides for 12 h. Brain tissue immunofluorescence staining After drying and reacting with blocking buffer (5% BSA) for 1 h at 25°C, the brain tissue slices were rinsed, and other materials such as neuronal nuclei (NeuN, Cat no. 94403, Cell Signaling Technology, Danvers, MA, USA), cluster of differentiation 68 (CD68, Cat no. ab283654, Abcam, Cambridge, UK), glial fibrillary acidic protein (GFAP, Cat no. 12389, Cell Signaling Technology), tumor necrosis factor-α (TNF-α, Cat no. ab183218, Abcam) and myelin-associated glycoprotein (MAG; Cat No. ab277524; Abcam) and myelin basic protein (MBP; Cat No. ab313827; Abcam, Cambridge, UK) antibodies were added. ..



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